Pig Interleukin 12(IL-12) ELISA Kit

Cat.#:

JL21867

Regulatory Status:

RUO

Alternative:

CLMF; CLMF p40; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL 12B; IL-12 subunit p40; IL-12B; IL12 p40; IL12B; IL12B_HUMAN; IMD28; IMD29; Interleukin 12 beta chain; Interleukin 12 p40; Interleukin-12 subunit beta; NK cell stimulatory fa

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.6-1000pg/mL

Sensitivity:

7.4pg/mL

Specificity:

This kit recognizes Pig IL-12 in samples. No significant cross-reactivity or interference between Pig IL-12 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| IL-12

Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells,macrophages, neutrophils, helper T cells and B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 belongs to the family of interleukin-12. IL-12 family is unique in comprising the only heterodimeric cytokines, which includes IL-12, IL-23, IL-27 and IL-35.Despite sharing many structural features and molecular partners, they mediate surprisingly diverse functional effects.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	1000	500	250	125	62.5	31.2	15.6	0
OD	2.39	1.67	1.17	0.71	0.52	0.33	0.27	0.1
Corrected OD	2.29	1.57	1.07	0.61	0.42	0.23	0.17	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Pig IL-12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-107	104

| Linearity

Samples were spiked with high concentrations of Pig IL-12 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	104-115
1 : 4	Average (%)	94	98	107